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Image Search Results
Journal:
Article Title: Isolation and Characterization of Two Proteins from Moraxella catarrhalis That Bear a Common Epitope
doi:
Figure Lengend Snippet: Interaction of proteins with fibronectin and vitronectin determined by dot blot. (A) Binding of vitronectin to UspA1 and UspA2. For this blot, the purified proteins were spotted onto the membrane, blocked, and incubated with human plasma vitronectin. The bound vitronectin was detected with a rabbit antivitronectin conjugate. (B) Binding of fibronectin to UspA1. Purified fibronectin was spotted onto the membrane, blocked, and incubated with purified UspA1. The bound UspA1 was then detected with MAb 17C7. (C) Binding of fibronectin to UspA2. This procedure was done as described for panel B except with UspA2. The amount of protein applied at each spot is indicated on the left.
Article Snippet: After the proteins had been absorbed into the membrane, it was blocked with BLOTTO for 1 h and incubated with
Techniques: Dot Blot, Binding Assay, Purification, Incubation